Làm thế nào một thiết bị năng lượng âm thanh siêu âm trong phòng thí nghiệm bảo tồn tính toàn vẹn của protein và axit nucleic

Introduction

In life science research, the integrity of extracted proteins and nucleic acids directly determines the reliability of downstream analyses such as next-generation sequencing (NGS), mass spectrometry, and qPCR. Yet traditional sample disruption methods—probe sonicators and water bath sonicators—often introduce thermal damage, contamination, and irreproducible fragmentation patterns .

The Lab Ultrasonic Sound Energy Device, particularly focused ultrasonication technology, offers a fundamentally different approach. This type of system has the ability to keep molecular structures intact and maintain isothermal conditions by focusing acoustic energy onto a sample while simultaneously carrying out lysis.

Here, we analyze how advanced focused ultrasound systems aid in the preservation of sensitive biomolecules. This is made possible by recent research as well as the advanced engineering from Công nghệ Longlight's BoFU-1600 Focused Ultrasonicator.

The Issue: Sonication and Biomolecule Damage

Using traditional ultrasound systems contributes three main problems to the preservation of proteins and nucleic acids:

•Thermal destruction: traditional ultrasound systems that are not focused produce hot spots due to the phenomenon of cavitation that results in rapid temperature increases. This leads to the denaturing of proteins and the activation of proteases and nucleases.

•Oxidative damage: systems that use probes rely on direct or close contact to the sample and in turn, produce metal ions and free radicals. This results in the modification of amino acid side chains and the oxidation of DNA nucleobases.

•Mechanical destruction of the sample: ultrasound systems that are not focused produce large acoustic energy and result in unpredictable sample fragmentation.

This leads to a negative impact on the analysis and interpretation of data, especially in the instance of precious and/or very small biomolecular samples. Examples include samples that are obtained from laser capture microdissection or circulating tumor cells.

What Distinguishes a Lab Ultrasonic Sound Energy Device?

A Class 4 Lab Ultrasonic Sound Energy Device that utilizes focused ultrasonication has a special design that optimally shapes high frequency, short wavelength sound waves and focuses them on the sample vessel. The design features multiple protective elements.

•The first feature is energy that is delivered without contact. Sound waves can propagate through a medium that is controlled and therefore can be sent to sealed tubes without contact, and without the contamination or debris that would be caused by a probe.

•Next is the isothermal operating environment. The sample zone can be maintained at a constant temperature with the controlling and eliminating of process heat that would flow to sensitive molecules.

•The device also encloses the sample throughout the process. This design eliminates the contamination of the sample due to the creation of aerosols and maintains a sealed environment for the sample that is needed for further applications.

Research Evidence: Focused Ultrasound and the Need for Integrity

Study of 2025: the work of Li, Liu, and the Molecular Mechanisms of Focused Ultrasound

The study that Li and Liu published in 2025 in Frontiers of Bioengineering and Biotechnology focuses on the bioeffects of focused ultrasound from a medical and laboratory perspective. They found that, when the parameters are kept below the inertial cavitation threshold, non-thermal effects such as acoustic streaming and stable cavitation are most always present.

[Frontiers | Focused ultrasound in modern medicine:

bioengineering interfaces, molecular effects, and clinical breakthroughs]

Focused ultrasound has been demonstrated to lys and to homogenize tissues and cells, all without the heating of the bulk solution, thus maintaining the integrity of proteins and the nucleic acids. This study has shown that if the focused ultrasound is delivered with the correct settings, the mechanical effects will be applied to the membrane of the cell and will facilitate the lysis of the membrane of the cell, while the intracellular macromolecules will remain intact.

2026 Study: Neurochemical Research on Protein Preservation via Ultrasound

A study published in Neuroscience Research in 2026 examined if focused ultrasound damaged the integrity of proteins in primary neuron cultures. The method involved low-intensity, pulsed focused ultrasound at 300 kHz for 10 minutes. Protein quantification using the Bradford assay and MTS-assay-based cell viability measurement were performed 24 hours after treatment. The study concluded that there were no observed differences in protein concentration.

[Sono-mechanical nanostructures-enabled sustained precise ultrasound brain stimulation | Nature Communications]

Neuronal somata and neuritic membranes appeared healthy, and there was no membrane blebbing or cell shrinkage. An important conclusion of the study is that focused ultrasound safely amplified intracellular calcium signaling, which is a measure of successful mechanical stimulation, and caused no notable protein degradation or structural damage. The study uses the term acoustic window to describe the range of ultrasound parameters that can safely be used for the study of non-destructive sample processing.

Công nghệ Longlight's BoFU-1600: Engineering Preservation into Practice

Longlight Technology has translated these research insights into a production-ready Lab Ultrasonic Sound Energy Device. The BoFU-1600 Focused Ultrasonicator incorporates specific design features that actively preserve protein and nucleic acid integrity:

• True low-temperature, constant-temperature control:

A high-sensitivity temperature sensing and closed-loop control system monitors the actual sample zone—not merely the water bath—and adjusts power delivery in real time. This prevents the thermal spikes that denature enzymes, fragment RNA, and bias DNA shearing profiles .

• Processing samples via contact-free acoustic medium:

Ultrasound focus at the sample-substrate interface eliminates contact with sample handling tools by positioning a coupling medium at the sample-substrate interface. Sample handling tools cause leaching of metal ions and sample contamination. Eliminating contact with sample handling tools preserves sample integrity for sensitive assays which include MALDI-TOF MS and LC-MS/MS.

• Acoustic confocal focusing:

Acoustic waves of a short wavelength focus with high precision. This technique minimizes losses outside the region of interest. It achieves maximum mechanical energy in a targeted area, resulting in rapid and efficient lysis of a sample.

• Recorded Processing Results:

Each run's processing parameters (power, time, temp, mode, etc.) are documented. This allows us to monitor an experiment and achieve GLP with minimal effort. Replications can take place regardless of the technician location or identity.

• Flexibility to Sample Size:

There are 16 sample positions. This allows custom sample processing conditions for up to 16 samples at a time. Rare or heterogeneous samples receive tailored acoustic exposure, preventing over-processing that would otherwise degrade limited material.

The Thermal Discipline Principle

Across genomics, proteomics, and clinical diagnostics, a consistent principle emerges: thermal control during sample disruption is the single most critical determinant of molecular integrity . Uncontrolled heating during sonication produces drifting fragment sizes in DNA shearing, loss of low-abundance proteins in proteomics, and inconsistent crosslink reversal in FFPE workflows.

The Lab Ultrasonic Sound Energy Device with focused, isothermal operation transforms an historically analog, variable process into a digitally controlled, reproducible method. By decoupling mechanical disruption from thermal stress, these systems enable researchers to obtain high-quality DNA, RNA, and protein from even the most challenging sample types—without compromising the very molecules they seek to analyze.

Kết thúc

For laboratories performing NGS library preparation, proteomic sample processing, or rare-cell analysis, the choice of disruption technology directly affects data quality and experimental reproducibility. Focused ultrasonication, as implemented in Longlight Technology's BoFU-1600, represents a significant advance over traditional probe and water bath systems. Supported by peer-reviewed research demonstrating preserved protein integrity and neuronal viability , these Lab Ultrasonic Sound Energy Device solutions offer a compelling path forward for sensitive biomolecule extraction.

*For technical specifications or to request a quote for the BoFU-1600 Focused Ultrasonicator, contact Longlight Technology.*

Câu hỏi thường gặp

Q1: How does the BoFU-1600 prevent thermal degradation of nucleic acids while DNAsing?

A: The BoFU-1600 uses Focused Ultrasonication while controlling for temperature in real time. This allows for shearing DNA without thermal degradation.

Q2: Does non-contact processing really reduce contamination risk?

A: Yes. In the BoFU-1600 when the ultrasonic probe is fully covered by the acoustic medium, there is no contact with the sample. This prevents cross contamination of the sample, as well as leakage of metals into the sample.

Q3: What sample types benefit most from focused ultrasound preservation?

A: FFPE sections, circulating tumor cells, primary neurons, filamentous fungi, and samples that are precious, heat-sensitive, or contamination-sensitive.

Q4: How does the BoFU-1600 guarantee reproducible results for different operators?

A: The BoFU-1600 is factory-calibrated and the built-in operating system records all parameters (power, time, temperature) for complete traceability and replication of the method.

Q5: Does the BoFU-1600 work for single-cell lysates, and samples that are of low-input?

A: Yes. The system is fully non-contact, low-volume, and energetic control of